Changes of microbial and metabolome of the equine hindgut during oligofructose-induced laminitis

Abstract Background Laminitis is a common and serve disease which caused by inflammation pathological changes of the laminar junction. However, pathologic mechanism remains unclear. In this study we aimed to investigate gut microbiota metabolomics in oligofructose-induced laminitis horses. Results Animals submitted treatment with oligofructose had lower fecal pH but higher lactic acid, histamine, Lipopolysaccharide (LPS) serum. Meanwhile, altered composition hindgut bacterial community, demonstrated increasing relative abundance Lactobacillus Megasphaera . addition, metabolome analysis revealed that decreased 84 metabolites while 53 increased, such as dihydrothymine, N3,N4-Dimethyl-L-arginine, 10E,12Z-Octadecadienoic asparagine. Pathway aldosterone synthesis secretion, regulation lipolysis adipocytes, steroid hormone biosynthesis, pyrimidine metabolism, biosynthesis unsaturated fatty acids, galactose metabolism were significantly different between healthy Furthermore, correlation indicated and/or positively associated Conclusions These results disturbance occurred during development equine laminitis, these may provide novel insights detect biomarkers for better understanding potential prevention strategies